立教大学 | 西池袋 | 関根研究室HP

研究業績

我らのボス、関根靖彦の主な研究業績です。
各論文は、NCBIのPubMedにリンクしています。

Odahara M, Sekine Y. (2018) RECX interacts with mitochondrial RECA to maintain mitochondrial genome stability. Plant Physiol. 2018 Mar 26. doi: 10.1104/pp.18.00218.

Sudo N, Soma A, Iyoda S, Oshima T, Ohto Y, Saito K, Sekine Y. (2018) Small RNA Esr41 inversely regulates expression of LEE and flagellar genes in enterohaemorrhagic Escherichia coli. Microbiology. 2018 Mar 26. doi: 10.1099/mic.0.000652.

Mitsunaka S, Sudo N, Sekine Y. (2017) Lysogenisation of Shiga toxin-encoding bacteriophage represses cell motility.J Gen Appl Microbiol. 2017 Dec 8. doi: 10.2323/jgam.2017.05.001.

Kobayashi Y, Misumi O, Odahara M, Ishibashi K, Hirono M, Hidaka K, Endo M, Sugiyama H, Iwasaki H, Kuroiwa T, Shikanai T, Nishimura Y. (2017) Holliday junction resolvases mediate chloroplast nucleoid segregation. Science. 2017 May 12;356(6338):631-634.

Odahara M, Kishita Y, Sekine Y MSH1 maintains organelle genome stability and genetically interacts with RECA and RECG in the moss Physcomitrella patens. Plant Journal (2017) Aug 91(3):455-465.

Odahara M, Kobayashi Y, Shikanai T, Nishimura Y Dynamic interplay between nucleoid segregation and genome integrity in Chlamydomonas chloroplasts. Plant Physiology (2016) Dec;172(4):2337-2346.

Odahara M, Inouye T, Nishimura Y and Sekine Y. (2016) PCR-based Assay for Genome Integrity after Methyl Methanesulfonate Damage in Physcomitrella patens. Bio-protocol (2016) 6(19): e1954. DOI: 10.21769/BioProtoc.1954

Odahara M, Inouye T, Nishimura Y, Sekine Y. RECA plays a dual role in the maintenance of chloroplast genome stability in Physcomitrella patens. Plant Journal (2015) Nov;84(3):516-26.

Odahara M, Masuda Y, Sato M, Wakazaki, M, Harada C, Toyooka K, and Sekine Y. (2015) RECG maintains plastid and mitochondrial genome stability by suppressing extensive recombination between short dispersed repeats. PLoS Genet 11(3):e1005080. doi:10.1371/journal.pgen.1005080

Ohnuma M, Yokoyama T, Inouye T, Sekine Y, Kuroiwa T, and Tanaka K. (2014) Optimization of polyethylene glycol (PEG)-mediated DNA introduction conditions for transient gene expression in the unicellular red alga Cyanidioschyzon merolae. J Gen Appl Microbiol., 60:156-9.

Suzuki S, Tanigawa O, Akanuma G, Nanamiya H, Kawamura F, Tagami K, Nomura N, Kawabata T, and Sekine Y. (2014) Enhanced expression of Bacillus subtilis yaaA can restore both the growth and sporulation defects caused by mutation of rplB, encoding ribosomal protein L2. Microbiology, 160:1040–1053.

Sudo N, Soma A, Muto A, Iyoda S, Suh M, Kurihara N, Abe H, Tobe T, Ogura Y, Hayashi T, Kurokawa K, Ohnishi M, and Sekine Y. (2014) A novel small regulatory RNA enhances cell motility in enterohemorrhagic Escherichia coli. J Gen Appl Microbiol. 60:44-50.

Soma A, Sugahara J, Onodera A, Yachie N, Kanai A, Watanabe S, Yoshikawa H, Ohnuma M, Kuroiwa H, Kuroiwa T, and Sekine Y. (2013) Identification of highly-disrupted tRNA genes in nuclear genome of the red alga, Cyanidioschyzon merolae 10D. Sci. Rep. 3:2321. doi: 10.1038/srep02321

Akanuma G, Nanamiya H, Natori Y, Yano K, Suzuki S, Omata S, Ishizuka M, Sekine Y, and Kawamura F. (2012) Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. J. Bacteriol. 194:6282-6291.

Imamura, S., Terashita, M., Ohnuma, M., Maruyama, S., Minoda, A., Weber, A. P. M., Inouye, T., Sekine, Y., Fujita, Y., Omata, T., and Tanaka, K. (2010) Nitrate assimilatory genes and their transcriptional regulation in a unicellular red alga Cyanidioschyzon merolae: Genetic evidence for nitrite reduction by a sulfite reductase-like enzyme. Plant & Cell Physiology, 51, 707-717.

Saito, T., Chibazakura, T., Takahashi, K., Yoshikawa, H., and Sekine, Y. (2010) Measurements of transposition frequency of insertion sequence IS1 by GFP hop-on assay. J. Gen. Appl. Microbiol. 56, 187-192.

Imamura, S., Kanesaki, Y., Ohnuma, M., Inouye, T., Sekine, Y., Fujiwara, T., Kuroiwa, T., and Tanaka, K. (2009) R2R3-type MYB transcription factor, CmMYB1, is a central nitrogen assimilation regulator in Cyanidioschyzon merolae. Proc. Natl. Acad. Sci. U S A, 106, 12548 12553.

Odahara M, Kuroiwa H, Kuroiwa T, Sekine Y. (2009) Suppression of repeat-mediated gross mitochondrialgenome rearrangements by RecA in the moss Physcomitrella patens. Plant Cell, 21, 1182 1194

Inouye, T., Odahara, M., Fujita, T., Hasebe, M., and Sekine, Y. (2008) Expression and complementation analyses of a chloroplast-localized homologue of bacterial RecA in the moss, Physcomitrella patens. Biosci. Biotechnol. Biochem., 72, 1340-1347.

Ohnuma, M., Yokoyama, T., Inouye, T., Sekine, Y., and Tanaka, K. (2008) Polyethylene glycol (PEG)-mediated transient gene expression in a red alga, Cyanidioschyzon merolae 10D. Plant & Cell Physiology, 49, 117-120.

Soma, A., Onodera, A., Sugahara, J., Kanai, A., Yachie, N., Tomita, M., Kawamura, F., and Sekine, Y. (2007) Permuted tRNA genes expressed via a circular RNA intermediate in Cyanidioschyzon merolae. Science, 318(5849):450-453.

Takahashi, K., Sekine, Y., Chibazakura, T., and Yoshikawa, H. (2007) Development of an intermolecular transposition assay system in Bacillus subtilis 168 using IS4Bsu1 from Bacillus subtilis (natto). Microbiology, 153, 2553-2559.

Terasawa, K,, Odahara, M., Kabeya, Y., Kikugawa, T., Sekine, Y., Fujiwara, M., and Sato, N.(2007) The Mitochondrial Genome of the Moss Physcomitrella patens Sheds New Light on Mitochondrial Evolution in Land Plants. Mol. Biol. Evol. 24, 699-709.

Takahashi, K., Chibazakura, T., Sekine, Y., Yoshikawa, H. (2007) Development of a new “GFP hop-on assay” system for insertion sequence transposition in Bacillus subtilis 168 using IS4Bsu1 from B. subtilis (natto). Biochem. Biophys. Res. Commun., 355, 426-30.

Odahara, M., Inouye, T., Fujita, T., Hasebe, M., and Sekine, Y. (2007) Involvement of mitochondrial-targeted RecA in the repair of mitochondrial DNA in the moss, Physcomitrella patens. Genes & Genetic Systems, 82, 43-51.

Sugahara, J., Yachie, N. Sekine, Y., Soma, A., Matsui, M., Tomita M., and Kanai, A. (2006) SPLITS: a new program for predicting split and intron-containing tRNA genes at the genome level. In Silico Biology, 6, 411-418.

Ikeuchi, Y., Soma, A., Ote, T., Kato, J. , Sekine, Y., and Suzuki, T. (2005) Molecular mechanism of lysidine synthesis that determines tRNA identity and codon recognition. Mol Cell. 19, 235-246.

Nakanishi, K., Fukai, S., Ikeuchi, Y., Soma, A. , Sekine, Y., Suzuki, T.,and Nureki, O. (2005) Structural basis for lysidine formation by ATP pyrophosphatase accompanied with a lysine-specific loop and a tRNA-recognition domain. Proc. Natl. Acad.Sci. USA,102, 7487-7492.

Ohtsubo, E., Minematsu, H., Tsuchida, K.,Choi, S., Ohtsubo, H., and Sekine, Y.(2004) Intermediate molecules generated by transposase in the pathway of transposition of bacterial insertion elementIS3. Adv. Biophys. 38,125- 139

Soma, A., Ikeuchi, Y., Kanemasa, S., Kobayashi, K., Ogasawara, N., Ote, T., Kato, J., Watanabe, K., Sekine, Y.,Suzuki,T. (2003) An RNA-modifying enzyme that governs both the codon and amino acid specificities of isoleucine tRNA. Mol. Cell. 12, 689-698.

Ohta, S., Tsuchida, K., Choi, S., Sekine, Y., Shiga, Y., Ohtsubo, E. (2002) Presence of a Characteristic D-D-E Motif in IS1 Transposase. J. Bacteriol. 184, 6146-6154

Urasaki, A., Sekine, Y. and Ohtsubo, E. (2002) Transposition of cyanobacterium insertion element ISY100 in Escherichia coli. J. Bacteriol. 184, 5104-5112.

Shiga, Y., Sekine, Y., Kano, Y. and Ohtsubo, E. (2001) Involvement of H-NS in transpositional recombination mediated by IS1.J. Bacteriol.183, 2476-2484

Inokuchi, Y., Hirashima, A., Sekine, Y., Janosi, L. and Kaji, A. (2000) Role of ribosome recycling factor (RRF) in translational coupling. EMBO J., 19, 3788-3798.

Janosi, L., Mori, H., Sekine, Y., Abragan, J., Janosi, R., Hirokawa, G. and Kaji, A. (2000) Mutations influencing the frr gene coding for ribosome recycling factor (RRF). J. Mol. Biol. 295, 815-829

Sekine, Y., Aihara, K., and Ohtsubo, E. (1999) Linearization and transposition of circular molecules of insertion sequence IS3. J. Mol. Biol. 294, 21-34.

Shiga, Y., Sekine, Y. and Ohtsubo, E. (1999) Transposition of IS1 circles. Genes to Cells, 4, 551-561.

Janosi, L., Mottagui-Tabar, S., Isaksson, L. A., Sekine, Y., Ohtsubo, E., Zhang, S., Goon, S., Nelken, S., Shuda, M. and Kaji, A. (1998) Evidence for in vivo ribosome recycling, the fourth step in protein biosynthesis. EMBO J., 17, 1141-1151.

Sekine, Y, Izumi, K., Mizuno, T. and Ohtsubo, E. (1997) Inhibition of transpositional recombination by OrfA and OrfB proteins encoded by insertion sequence IS3. Genes to Cells, 2, 547-557.

Sekine, Y., Eisaki, N., Kobayashi, K. and Ohtsubo, E. (1997) Isolation and characterization of IS1 circles. Gene, 191, 183-190. required for production of thetransposase encoded by insertion sequence 1.Proc. Natl. Acad. Sci. USA, 86, 4609-4613.

Sekine, Y., Eisaki, N. and Ohtsubo, E. (1996)Identification and characterization of the linear IS3 molecules generated by staggered breaks.J. Biol. Chem., 271, 197-202.

Ohtsubo, E. and Sekine, Y. (1996)Bacterial insertion sequences. Cur. Topics Microbiol. Immunol., 204, 1-26.

Sekino, N., Sekine, Y. and Ohtsubo, E. (1995)IS1-encoded proteins, InsA and the InsA-B'-InsB transframe protein (transposase): Functions deduced from their DNA-binding ability. Adv. Biophys., 31, 209-222.

Sekine, Y., Eisaki, N. and Ohtsubo, E. (1994)Translational control in production of transposase and in transposition of insertion sequence IS3. J. Mol. Biol., 235, 1406-1420.

Sekine, Y and Ohtsubo, E. (1992) DNA sequences required for translational frameshifting in production of transposase encoded by IS1.Mol. Gen. Genet., 235, 325-332.

Sekine, Y and Ohtsubo, E. (1992) DNA sequences required for translational frameshifting in production of transposase encoded by IS1.Mol. Gen. Genet., 235, 325-332.

Sekine, Y., Nagasawa, H. and Ohtsubo, E. (1992)Identification of the site of translational frameshifting requierd for production of the transposase encoded by insertion sequence IS1. Mol. Gen. Genet., 235, 317-324.

Sekine, Y. and Ohtsubo, E. (1991) Translational frameshifting in IS elements and other genetic systems. pp. 243-261. In: New Aspects of The Genetics of Molecular Evolution, Edited by Kimura, M. and Takahata,N.,Japan Scientific Societies Press, Tokyo / Springer-Verlag, Berlin.

Sekine, Y and Ohtsubo, E. (1989)Frameshifting is required for production of the transposase encoded by insertion sequence 1.

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